Studies on the Lipolytic Enzyme of Molds -Part. 4. Characteristics of the Purified lipase of Rhizopus Japonicus-

사상균(絲狀菌)의 지방분해효소(脂肪分解酵素)에 관(關)한 연구(硏究) -제(第)4보(報) 분리사상균(分離絲狀菌) Rhizopus japonicus의 정제(精製) Lipase의 특성(特性)에 관(關)하여-

These experiment were conducted to investigate the characteristics of the purified lipase of Rhizopus japonicus. 1. The optimum pH for the lipase action was 6.8, the stable pH ranged $5.0{\sim}8.0$ and the optimum temperature was $35^{\circ}C$. The Purified lipase was stable below $45^{\circ}C$ and inactivated abruptly above $45^{\circ}C$ 2. Coconutoil castor oil and palm oil were most favorably hydrolyzed by the purified lipase, wheres olive oil and soybean oil were slightly less than the former three, and Tween 80 was hydrolyzed only 30% compared with coconut oil. 3. The purified lipase was activated by $Ca^{++}$ and $Mg^{++}$, whereas it was inhibited by $Hg^{++}$ and $Fe^{++}$.

Rhizopus japonicus의 정제(精製) lipase의 특성(特性)을 검토(檢討)한 결과(結果)를 요약(要約)하면 다음과 같다. 1. 정제(精製) lipase의 작용최적(作用最適) PH는 6.8, 안정(安定) PH 범위(範圍)는 $5.0{\sim}8.0$, 최적온도(最適溫度)는 $35^{\circ}C$이고, $45^{\circ}C$이하(以下)에서 안정(安定)하였으며 그 이상(以上)의 온도(溫度)에서는 급격(急激)하게 불활성화(不活性化) 되었다. 2. 정제(精製) lipase는 coconut oil, castor oil 및 palm oil을 가장 잘 분해(分解)시켰다. olive oil, 및 soybean oil에서는 분해율(分解率)이 약간 떨어지며, Tween 80에서는 분해율(分解率)이 coconut oil의 30% 정도(程度) 밖에 되지 않았다. 3. 정제(精製) lipase는 $Ca^{++}$, $Mg^{++}$에 의(依)하여 현저(顯著)하게 활성화(活性化)되었으며, $Hg^{++}$ $Fe^{+++}$에 의(依)하여는 심(甚)하게 저해(沮害)되었다.