Antioxidant Activity of Methylene Chloride Extracts Obtained from Glucose-Ammonia (1M+8M) Browning Mixtures

Glucose-Ammonia (1M+8M) 갈색화(褐色化) 반응액(反應液)에서 얻어진 Methylene Chloride 추출물(抽出物)의 항산화(抗酸化) 효과(?果)

An attempt was made to investigate the antioxidant activity of methylene chloride extracts of a glucose-ammonia browning reaction mixture, which contain pyrazines, important intermediate prod ucts of Maillard-type browning reactions. Methylene chloride extracts were obtained from a glucose-ammonia(1M+8M) browning reaction mixture, which had been heated at $100^{\circ}C$. for 4 hours. The molar ratio of the reactants and the reaction time were reported to be the optimum ratio and time for the maximum formation of pyrazines. The methylene chloride extracts and furfural (for comparison purpose) were added to edible rapeseed oil substrates, and the resulting substrates and the control were stored at $37.0{\pm}1.0^{\circ}C$. Peroxide values (POV), thiobarbituric acid values (TBA-values) and acid values (AV) of the substrates and the control were determined regularly during a 34-day storage period. The antioxidant activity of the methylene chloride extracts and furfural was estimated on the basis of POV, TBA-value and AV-development of the substrates and the control. It was found that the methylene chloride extracts of the glucose-ammonia (1M+8M) browning mixture possessed considerable antioxidant activity. Furfural also showed some activity.

본실험(本實驗)에서는 Maillard형(型) 갈색화반응(褐色化反應)의 중요(重要)한 중간생성체(中間生成體)의 하나인 pyrazine유도체(誘導體)의 항산화효과(抗酸化效果)를 보기위해 먼저 이를 주요구성성분(主要構成成分)으로 하는 methylene chloride추출물(抽出物)의 항산화효과(抗酸化效果)를 검토(檢討)하고저 했다. 즉, pyrazine유동체생성(誘導體生成)의 최적조건(最適條件)에 따라 glucose-ammonia(1M+8M)갈색화(褐色化) 반응액(反應液)을 $100^{\circ}C$에서 4시간(時間) 반응(反應)시킨후, 여기서 methylene chloride추출물(抽出物)을 얻었다. 이 추출물(抽出物)과 별도(別途)로 furfural을 식용(食用)평지씨기름기질(基質)에 첨가(添加)하여, control과 함께 $37^{\circ}C{\pm}1.0^{\circ}C$에서 저장(貯藏)하여, 그 과산화물가(過酸化物價)(POV), thiobarbituric acid value(TBA 가(價))와 산가(酸價)(AV)를 규칙적으로 측정(測定)하였다. 그리고 methylene chloride추출물(抽出物)과 furfural이 들은 기질(基質)들의 POV, TBA가(價)와 AV의 변화(變化)를 control의 그것과 비교(比較)하여 methylene chloride추출물(抽出物)과 furfural의 항산화효과(抗酸化效果)를 검토(檢討)하였다. 이상(以上)의 실험(實驗)의 결과(結果), glucose-ammonia (1M+8M)갈색화반응액(褐色化反應液)의 methylene chloride추출물(抽出物)에는 상당(相當)한 항산화효과(抗酸化效果)를, 그리고 furfural에도 다소의 항산화효과(抗酸化效果)를 확인(確認)할 수 있었다.