Decomposition of Guanosine-5’-Monophosphate by Heat Treatment

구아닌 산의 열(熱) 분해(分解)에 관(關)한 연구(硏究)

The heat decomposition rate of guanosine-5'-monophosphate was investigated in pH range from 5.52 to 7.00, and 0.1 of the ionic strength. The result showed that the rate was a first-order reaction and the rate of guanosine-5'-monophosphate loss was maximum near $pKa_2$. The loss of guanosine-5'-monophosphate was temperature dependent and followed to the Arrhenius equation in the temperature region from $93^{\circ}C$ to $108^{\circ}C$. The rate constant as function of temperature ($93^{\circ}C$ to $108^{\circ}C$) and neutral pH($pKa_2$, 6.0, to 7.0) was correlated by least-square fit of the experimental data; $$K=4.19{\times}10^{26}\;{\exp}\;[-1.3(pH+E/RT)]$$

5-GMP의 열 분해 반응을 pH 5.52에서 7.00의 범위에서 조사하였다. 그 반응 속도는 일차 반응으로 나타났으며, 용액의 pH가 $pKa_2$일 때 반응속도가 가장 컸다. 5'-GMP의 분해 반응은 온도 의존성을 나타냈으며, $93^{\circ}C$에서 $108^{\circ}C$ 범위에서 Arrhenius식을 따르고 있었고, 반응 속도 상수는 $pKa_2$ 이상의 중성 용액 근처에서는 온도 및 pH의 함수로서 다음과 같이 표시되었다. $$K=4.19{\times}10^{26}\;Exp[-1.3(pH+E/RT)]$$