Abstract
The model compounds of Vitamin $B_1$ 3-benzyl-4-methylthiazolium bromide, (1), 3, 4-dimethylthiazolium iodide (2) and 2-($\alpha$-hydroxyethyl)-3-benzyl-4-methylthiazolium bromide (3) were prepared by the organic synthesis. Colorimetric determination with phosphotungstic acid showed an increase of $1.1{\sim}1.2$ folds with compound (1) and $1.5{\sim}1.9$ folds with compound (3) when readings were carried out after an overnight, compared with immediate readings. Colorimetry with 2.6-dibromoquinone chloroimide yielded the compound (1) being $2.2{\sim}2.5$ folds higher than the compound (3). The half wave potentials and diffusion currents of anodic and cathodic waves of polarography with the same concentration of the compounds (1), (2) and (3) also resulted in different values of their waves. Therefore, it was a firm conclusion that any values obtained from quantitative analysis with this model compounds (1), (2) and (3) were not directly applicable to those of $B_1$.
Vitamin $B_1$의 모형(模型) 화합물(化合物)인 3-benzyl-4-methylthiazolium bromide, (1), 3, 4-dimethylthiazolium iodide (2) and 2-($\alpha$-hydroxyethyl)-3-benzyl-4-methylthiazolium bromide (3)을 유기(有機) 화학적(化學的) 합성법(合成法)으로 제조(製造)하였다. Thiazole 유도체(誘導體)는 phosphotungstic acid로 발색(發色)시켜 하룻밤 방치(放置) 후(後)의 흡광도(吸光度)가 발색(發色) 즉시(卽時)의 흡광도(吸光度)에 비(比)하여 화합물(化合物)(1)은 $1.1{\sim}1.2$배(倍) 화합물(化合物)(3)은 $1.5{\sim}1.9$배(倍)가 증가(增加)하였다. 2.6-dibromoquinone chloroimide 비색(比色) 정양법(定量法)은 화합물(化合物)(1)이 화합물(化合物)(3)에 비(比)하여 $2.2{\sim}2.5$배나 늘었다. 화합물(化合物)(1), (2), (3)의 산화파(酸化波) 그리고 환원파(還元波)의 half wave potential과 확산(擴散) 전류(電流)는 모두 그 값이 다르다. 이 연구(硏究)의 결과(結果)로 볼 때 이 모형(模型) 화합물(化合物)(1), (2), (3)의 정량가(定量價)를 그대로 Vitamin $B_1$에 응용(應用)할 수 없음이 판명(判明)되었다.