Abstract
Equal portions of a Maillard-type browning mixture (0.2 M glucose+0.2 M glycine), heated at $100^{\circ}C$ for 12 hr, were extracted with the same amounts of eight solvents, respectively. The extracts were then dissolved in equal amounts of an edible soybean oil, and the resulting substrates and a portion of the soybean oil (Control) were stored in an incubator kept at $45.0{\pm}1.0^{\circ}C$ for three weeks. Peroxide values and TBA values of Control and the substrates were determined regularly during the storage period. The POVs of Control and the substrates containing acetone, benzene, chloroform, ethanol, diethyl ether, methanol, methylene chloride, and petroleum ether extracts after 12 days of storage were respectively $60.0{\pm}3.6$, $31.9{\pm}0.9$, $37.6{\pm}2.2$, $48.1{\pm}1.1$, $11.9{\pm}1.3$, $4.85{\pm}0.4$, $11.5{\pm}1.0$, $45.3{\pm}0.3$, and $43.3{\pm}4.2\;m.\;mole/kg\;oil$. The TBA values after 16 days of storage were respectively $0.28{\pm}0.02$, $0.20{\pm}0.01$, $0.21{\pm}0.01$, $0.26{\pm}0.03$, $0.16{\pm}0.02$, $0.28{\pm}0.02$, $0.17{\pm}0.01$, $0.33{\pm}0.05$, and $0.31{\pm}0.02$. The induction periods (arbitrarily taken as the time in hours for a substrate to reach a peroxide value of 30 m. mole/kg oil) of Control and the substrates were respectively 193, 280, 252, 220, 478, 229, 455, 217, and 214 hr. The antioxidant activity of each extract estimated on the basis of the length of the induction periods was, in decreasing order, as follows; ethanol>methanol>acetone>benzene>diethyl ether>chloroform, pertroleum ether, methylene chloride.
$100^{\circ}C$에서 12시간 가열한 Maillard형(型) 갈색화반응액(褐色化反應液)을 8개의 용매(溶媒)로 추출하여 각 추출물(抽出物)의 산화억제(酸化抑制) 작용(作用)을 대두유(大豆油)를 기질(基質)로 하여 비교하고자 하였다. 실험대조(實驗對照)와 아세톤, 벤젠, 클로로포름, 에탄올, 에테르, 메탄올, 메틸렌 클로라이드, 석유에테르 추출물(抽出物)이 들은 기질(基質)의 저장 12일 후의 과산화물(過酸化物)값, 16일 후의 TBA값은 각각 66.0, 31.9, 37.6, 48.1, 11.9, 48.5, 11.5, 45.3, 43.3 (m. mole/kg oil)과 0.28, 0.20, 0.21, 0.26, 0.16, 0.28, 0.17, 0.33, 0.31이었다. 과산화물(過酸化物)값이 30이 되는 데 소요한 시간을 유도기간(誘導期間)으로 할 때 실험대조(實驗對照)와 각용매추출물(各溶媒抽出物)이 들은 기질(基質)의 유도기간(誘導期間)은 각각 193, 280, 252, 220, 478, 229, 455, 217과 214시간이었다.