Streptomyces의 Aerial Mycelium 형성에 대한 Glucose 억제 기작에 관한 연구

The Glucose Repression of Aerial Mycelium Formation in Streptomyces

We have demonstrated that both L-histidine as an amino acid factor and dextrin as a carbon source were required for the glucose repression. 1% glucose was sufficient to the glucose repression of aerial mycelium formation in Streptomyces lavendulae and Streptomyces aureofacience. the synthesized medium, KK, which is lack of all orgnic nutrients except dextrin was able to induce glucose repression, but the addition of 0.003% or more L-histidiner recovers the capacity of glucose repression. 0.02% or more histidine was reuqired for glucose repression of aerial mycelium formation in the absence of dextrin. Treatments of $5{\mu}M$ ormore ethidium bromide (EtBr0 gave rise to bald mutants at high frequency in Streptomyces aureofaciens, and it is probable that the gene(s) for the function of aerial mycelium formation is linked to plasmed DNA in this species.