Study on the Microbial Degradation of Nicotine (1) -Isolation and Identification of Nicotinophiles

미생물에 의한 니코틴 분해 연구(1) -니코틴 분해세균의 분리 및 동정

From 143 sources of collected samples, the distribution of nicotinophiles were investigated and the biological characteristics as well as the rate of nicotine degradation were determined for the selected 34 strains which could grow successfully in the nicotine media, and one of the most effective strains was chosen and identified at the species level. Nicotinophils were distributed abundantly in the soils rich with organic materials, tobacco seed and root. The selected 34 strains were classified into 7 genus and identified with 4 strains of Arthrobacter, 11 strains of non-pigmented Pseudomonas, 2 strains of pigmented Pseudomonas, 6 strains of Alkaligenes, 5 strains Chromobacter 2 strains of Listeria and 4 strains of Achromobacter. Pseeudomonas and Alkaligenes were better than other genus in the rate of nicotine degradation and tobacco seed and root were also good sources for the isolation of effective nicotinophiles. Amnog 34 strains, strain NCT 27 which exhibited 97.l% of nicotine degradation rate was the best one for nicotine degradation and was indentified with Pseudomonas putida.

143개의 여러가지 시료에서 니코틴 분해세균의 분포를 조사하고 니코틴 배지에서 생장능이 우수한 34균주를 선별하여 균주의 생물학적 특성과 니코틴 분해력을 조사함과 동시에 니코틴 분해력이 가장 우수한 균주를 선정하여 동정하였다. 니코틴 분해세균은 유기물이 풍부한 토양과 담배씨, 부리에 많이 분포되어 있었고 선별된 34균주는 Arthrobacter 4주, Pseudomonas (non-pigmented) 11주, Pseudomonas (Pigmented) 2주, Alkaligenes 6주, Chromobacter 5주, Listeria 2주, Achromobacter 4주 등으로 분류되었다. 니코틴 분해력에 있어서는, Pseudomonas와 Alkaligenes가 다른 속(genus)보다 높고, 서식지 별로 비교해 보면 담배씨와 뿌리에서 분리된 균주가 우수했으며 가장 우수한 균주는 97.l%의 분해율을 나타내는 균주번호 NCT27로서 Pseudomonas putida로 동정되었다.