분만전후의 어미쥐의 영양부족이 새끼쥐의 뇌성장발육과 조성에 미치는 영향

Effect of Maternal Undernutrition on the Growth and Composition of Young Rat Brain

분만전후 4주동안 Sqraque Dawley 암컷 쥐에게 양적으로 식이를 제한했다. 임의로 먹인 control group의 1일평균성취량의 1/2의 시중사로를 식이제한 group에게 주었다. Deficient I group에서는 분만후부터 이유시까지 식이제한을 했고, deficient II group에서는 임신 15일부터 이유시까지 계속 식이제한을 했다. 식이제한 group의 체중과 뇌무게는 control group의 새끼쥐보다 유의적으로 낮았지만, 체중에 대한 뇌무게의 비는 control group보다 높았다. 이유시에는 두 식이제한 group사이에서 체중과 뇌무게의 유의적인 차가 나타났다. 뇌의 DNA, RNA 및 단백질함량은 control group보다 식이제한 group에서 유의적으로 낮았지만 RNA/DNA, 뇌무게/DNA 및 단백질/DNA은 control group보다 식이제한 group에서 높았다. 이것은 새끼쥐뇌에서 세포분열이 세포의 크기성장보다 이 기간중의 어미쥐의 식이제한에 의해 더욱 심한 영향을 받았음을 시사해준다. 뇌의 DNA와 RNA는 두식이제한 group간에 유의적인 차를 나타내지만, 단백질의 경우는 유의적인 차를 나타내지 않았다.

A quantitative restriction of maternal diet without changes in quality of diet was given to the Sprague Dawley rats during the third week of gestation and lactation. Half the normal average daily intake of control group was given to deficient groups in this period. Female rats of control group were fed a commercial diet ad libitum throughout the experimental period. Dietary restriction started from birth to weaning in deficient I group and from the 15th day of gestation to weaning in deficient II group. Body and brain weight of offsprings of deficient groups were significantly lower than control group, but the ratios of brain weight to body weight in deficient groups were higher than the control group. Significant difference between deficient groups (I and II) was noticed at weaning. Brain DNA, RNA and total protein of offsprings of deficient groups were significantly lower than control group, but RNA/DNA, brain weight/DNA, and total protein/DNA show that cell number were more affected than the cell size by the maternal dietary restriction during the third week of gestation and lactation. Between the deficient groups, there was a significant difference in brain DNA and RNA, but no significant difference in total brain protein. (This research was supported in part by grant from the Ministry of Education.)