Studies on the Enzymatic Properties of Rice Bran Lipase

미강 Lipase의 효소 화학적 성질에 대한 연구

  • Choi, Mi-Ja (Dept. Home Economics, Gradnate School, Keimyung University) ;
  • Lee, Gui-Ju (Dept. Home Economics, Korea University)
  • Published : 1981.09.30

Abstract

Some enzymatic properties of the lipase extracted from rice bran were studied. The rate of free fatty acid formation as a function of incubation time was maximum after 30 min. at $35^{\circ}C$. The activation energy of the enzyme was 1.83 Kcal/mol and using a olive oil emulsion as substrate, the Km value was $0.8{\times}10^{-3}M$. The enzyme activity was more stimulated by calcium ion. Application of these enzymatic properties onto the effective utilization of rice bran and preservation of rice were discussed.

미강으로부터 추출한 lipase의 몇가지 효소 화학적 성질을 알아 보았다. 반응시간에 따른 유리 지방산의 생성율은 $35^{\circ}C$에서 30분후에 가장 높았다. 효소의 활성화 에너지는 1.83kca1/mo1e이였으며, olive oil emulsion을 기질로 사용하였을때 Km값은 $0.8{\times}10^{-3}mol/{\ell}$였다. 효소 활성은 Ca++이온에 의해 보다 촉진되었다. 미강의 효율적 이용 및 찰의 저장에 대한 이들 효소화학적 성질의 응용성이 토의 되었다.

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