Studies on the Triglyceride Composition of Some Vegetable Oils -I. Triglyceride Composition of Cotton Seed Oil-

식물유(植物油)의 Triglyceride 조성(組成)에 관한 연구(硏究) -제(第) 1 보(報) : 면실유(棉實油)의 Triglyceride 조성(組成)-

  • Choi, Su-An (Dept. of Scientific Education, Busan Teacher's College) ;
  • Park, Yeung-Ho (Dept. of Food Science and Technology, National Fisheries University of Busan)
  • 최수안 (부산교육대학 과학교육과) ;
  • 박영호 (부산수산대학 식품공학과)
  • Published : 1982.09.30

Abstract

Triglycerides of cottonseed oil were separated by thin layer chromatography (TLC), and fractionated by high-performance liquid chromatography (HPLC) on the basis of partition numbers. From each fraction, it was fractionated again on the basis of acyl carbon numbers using gas liquid chromatography (GLC). The fatty acids of triglyceride for each partition number group were analyzed by GLC. From, these results, triglyceride constituents of cotton seed oil were estimated to be 37 kinds of triglycerides. The major triglycerides and their contents in cotton seed oil were as follows: 25.8%$(C_{16:0},\;C_{18:2},\;C_{18:2})$, 15.5%$(C_{18:2},\;C_{18:2},\;C_{18:2})$, 13.8%$(C_{16:0},\;C_{18:2},\;C_{16:0})$, 8.3%$(C_{18:2},\;C_{18:1},\;C_{18:2})$, 6.2%$(C_{18:2},\;C_{18:1},\;C_{18:1})$, 4.1%$(C_{18:1},\;C_{18:1},\;C_{14:0})$, 3.4%$(C_{16:0},\;C_{18:1},\;C_{16:0})$, 2.3%$(C_{18:1},\;C_{18:2},\;C_{16:0})$, 2.2%$(C_{18:1},\;C_{18:1},\;C_{18:1})$, 1.0%$(C_{14:0},\;C_{18:2},\;C_{18:1})$.

식물유(植物油)의 트리-글리세리드의 조성(組成)을 밝히기 위하여 목화씨기름을 시료(試料)로 하여 TLC로써 트리-글리세리드를 분리하고 HPLC에 의하여 PN별(別)로 분획(分劃)하였으며, 각 획분(劃分)을 분취(分取)하여 GLC로 acyl탄소수별(別)로 분획(分劃) 하였다. 또한 PN별(別) 분획(分劃)을 GLC로 지방산 조성을 분석하였다. 이들 결과(結果)로부터 트리-글리세리드 조성을 산정하였는데, 목화씨기름에 있어서는 37종류이었다. 시료유(試料油)의 주요(主要) 트리-글리세리드를 들면 목화씨기름에 있어서는 25.8%$(C_{16:0},\;C_{18:2},\;C_{18:2})$, 15.5%$(C_{18:2},\;C_{18:2},\;C_{18:2})$, 13.8%$(C_{16:0},\;C_{18:2},\;C_{16:0})$, 8.3%$(C_{18:2},\;C_{18:1},\;C_{18:2}$), 6.2%($C_{18:2},\;C_{18:1},\;C_{18:1})$, 4.1%$(C_{18:1},\;C_{18:1},\;C_{14:0})$, 3.4%$(C_{16:0},\;C_{18:1},\;C_{16:0})$), 2.3%$(C_{18:1},\;C_{18:2},\;C_{16:0}$), 2.2%($C_{18:1},\;C_{18:1},\;C_{18:1})$, 1.0%$(C_{14:0},\;C_{18:2},\;C_{18:1})$이었다.

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