Studies on the Triglyceride Composition of Some Vegetable Oils -II. Triglyceride Composition of Corn Oil-

식물유(植物油)의 Triglyceride 조성(組成)에 관한 연구(硏究) -제(第) 2 보(報) : 옥수수유(油)의 triglyceride 조성(組成)-

  • Choi, Su-An (Dept. of Scientific Education, Busan Teacher's College) ;
  • Park, Yeung-Ho (Dept. of Food Science and Technology, National Fisheries University of Busan)
  • 최수안 (부산교육대학 과학교육과) ;
  • 박영호 (부산수산대학 식품공학과)
  • Published : 1982.09.30

Abstract

In order to define triglyceride compositions in fat and oil triglycerides were separated by thin layer chromatography (TLC) from corn oil, and the separated triglycerides were graduated according to each partition number(PN) by high performance liquid chromatography (HPLC) using column of ${\mu}-Bondapack\;C_{18}$ and each graduation was graduated again according to acylcarbon number by gas liquid chromatography(GLC). Fatty acid compositions were analyzed by GLC after their graduation were methylated in according to PN. The triglyceride compositions were estimated by synthesizing the above three results. The estimated triglycerides consisted of 36 kinds in corn oil. The major triglyceride compositions of sample oil were as follows: 21.5%$(C_{18:2},\;C_{18:2},\;C_{18:1})$, 17.4%$(C_{18:1},\;C_{18:2},\;C_{18:1})$, 15.4%$(C_{18:1},\;C_{18:2},\;C_{16:0})$, 11.1%$(C_{16:0},\;C_{18:2},\;C_{18:2})$, 9.0%$(C_{18:1},\;C_{18:1},\;C_{18:1})$, 8.0%$(C_{18:2},\;C_18:2},\;C_{18:2})$, 5.7%$(C_{18:1},\;C_{18:1},\;C_{16:0})$, 2.2%$(C_{16:0},\;C_{16:0},\;C_{18:2})$, 1.6%$(C_{18:2},\;C_{18:2},\;C_{18:2})$, 1.1%$C_{18:2},\;C_{18:0},\;C_{16:0})$, 1.1%$(C_{16:0},\;C_{16:0},\;C_{18:1})$.

주요 식물유(植物油)의 트리-글리세리드 조성(組成)을 구명(究明)하기 위하여 옥수수유(油)를 시료로 하여 TLC로서 트리-글리세리드를 분리하고 HPLC에 의하여 PN별(別)로 분획(分劃)하였으며, 각 획분(劃分)을 분취(分取)하여 GLC로 acyl탄소수별(炭素水別)로 분획(分劃) 하였다. 또 PN별(別) 획분(劃分)을 GLC로 지방산 조성을 분석하였다. 위의 3가지 방법으로부터 트리-글리세리드 조성(組成)을 산정한 종류는 36종류이었다. 시료유(試料油)의 주요 트리-글리세리드를 들면 다음과 같다. 21.5%$(C_{18:2},\;C_{18:2},\;C_{18:1})$, 17.4%$(C_{18:1},\;C_{18:2},\;C_{18:1})$, 15.4%$(C_{18:1},\;C_{18:2},\;C_{16:0})$, 11.1%$(C_{16:0},\;C_{18:2},\;C_{18:2})$, 9.0%$(C_{18:1},\;C_{18:1},\;C_{18:1})$, 8.0%$(C_{18:2},\;C_{18:2},\;C_{18:2})$, 5.7%$(C_{18:1},\;C_{18:1},\;C_{16:0})$, 2.2%$(C_{16:0},\;C_{16:0},\;C_{18:2})$, 1.6%$(C_{18:2},\;C_{18:2},\;C_{18:2})$, 1.1%$(C_{18:2},\;C_{18:0},\;C_{16:0})$, 1.1%$(C_{16:0},\;C_{16:0},\;C_{18:1})$이었다.

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