The Composition of Candida guilliermondii Grown in Soybean Whey

Soybean Whey에 배양(培養)한 Candida guilliermondii의 균체조성(菌體組成)

  • Lee, Kang-Ja (College of Home Economics, Yeungnam University)
  • Published : 1982.03.01

Abstract

A strain of yeast (Candida guilliermondii KFCC 35120) was cultivated by using the soybean whey with supplement of blackstrap molasses (4%) and ammonium acetate (0.5%). By the cultivation continued for 48 hours, 8.1g of dry cell was produced. The cell was consisted of carbohydrate 39.3%, protein 40.5%, lipid 3.9%, nucleic acid 9% and ashes 6%. The protein was analyzed to contain 18% of glutamic acid and 10% of lysine. Other amino acids showed a content near that of FAO reference protein except Methionine. Total lipid content was varied by nutritional condition especially by the kind of carbon source, however it showed a content 1.7 to 2.5 times higher than that of Saccaromyccs cerevisiae. The lipid contain 48% of oleic acid, 17% linoleic acid and 4% linolenic acid. The content of linoleic and lonolenic acid was higher than that of S.cerevisiae by 9 and 24 times, respectively.

두부(豆腐) 제조시(製造時) 방류(放流)되는 soybean whey에 4%의 폐밀당(廢糖密) 및 0.5% ammonium acetate 를 첨가(添加)하여 Candida guilliermondii(KFCC 35120)를 배양(培養)하여 liter 당(當)8.1g의 건조균체(乾燥菌體)를 얻었으며 균체(菌體) 분석(分析) 결과(結果)는 다음과 같다. 1. 건조균체(乾燥菌體)는 회수화물(灰水化物) 39.3%, 단백질(蛋白質) 40.5%, 지질(脂質) 3.9%, 핵산(核酸) 9%, 회분(灰分) 6%를 함유(含有)하였다. 2. Amino acid 조성(組成)은 lysine 함량(含量)이 약(約) 10%이었고 glutamic acid가 18%로서 가장 높은 함량(含量)을 나타내었다.3. 지방함량(脂肪含量)은 C. guilliermondii가 대조(對照)의 S. cerevisiae 보다 $1.7{\sim}2.5$배(倍) 높은 함량(含量)을 나타내었으며 양균주(兩菌株)가 모두 whey 단독(單獨)으로 培養한 균체(菌體)가 廢糖蜜을 첨가(添加)한 것 보다 약(約) 2배(倍)의 높은 함량(含量)을 나타내었다. 균체(菌體)의 지방산(脂肪酸) 조성(組成)은 oleic acid가 48%로서 가장 높은 함량(含量)을 나타내었고, linoleic acid와 linolenic acid 함량(含量)이 각각(各各) 17% 및 4%로서 S. cerevisiae에 비(比)하여 각각(各各) 9배(倍)와 24배(倍)에 대(達)한 반면(反面)에 palmitic acid 함량(含量)은 약(約) 5.5%로서 S. cerevisiae에 비(比)하여 낮았다.

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