Primary Screening for Growth Inhibitors of L1210 Cells from Oriental Herbs.

한약재로부터 L1210 세포 생장 억제물질의 검색

In order to obtain anticancer substances from natural products, extracts of dry herbs, which have long been used to treat cancer or canter-like diseases in oriental countries, were screened. Extracts were made with hot water and/or organic solvents. With the extracts we treated murine leukemic L1210 cells growing in Fischer's medium. After 48 hours of incubation, cells were counted and concentrations of dry extracts to achieve 50 percent inhibition of the control growth, ED$_{50}$ values, were determined. Among the 38 species of medicinal plants tested, water extracts of six species showed ED$_{50}$ values of substantially low. Further extraction with organic solvents could reduce their ED$_{50}$ values within the range of the NCI quality control limit. The promising species as potential sources of anti-cancer substances included Cinnamomum cassia, Citrus trifoliata, Coptis japonica, Panax ginseng, Phellodendron amurense, and Scutellaria baikalensis.

천연물 가운데서 항암물질을 얻을 목적으로 동양에서 오랫동안 암이나 암에 비슷한 질병의 치료에 씌여온 한약재를 물이나 유기용매로서 추출하여 검색하였다. Fischer배지에 자라는 L1210 세포에 추출물을 첨가한 다음 48시간 후에 세포수를 헤아려 생장을 50% 억제할 수 있는 추출물의 농도를 나타내는 ED$_{50}$값을 측정하였다. 물로서 추출한 38종의 한약재 가운데서 6종이 낮은 ED$_{50}$값을 나타냈다. 이들을 다시 유기용매로서 추출하므로써 ED$_{50}$값이 더욱 떨어져 NCI의 품질관리범위 치역내에 들어갔다. 항암제원으로 유망시되는 한약재로서 Cinnamomum cassia, Citrus trifoliata, Coptis japonica, Panax ginseng, Phellodendron amurense, Scutellaria baikalensis를 들 수가 있다.