Effect of Various Carbon Sources on Heterotrophic Acetylene Reducing Activities of Submerged Soil

담수토양(湛水土壤)에서 수종유기물(數種有機物)의 시용(施用)이 타양성(他養性) 질소고정력(窒素固定力)에 미치는 영향(影響)

The glucose application remarkably increased the heterotrophic acetylene reducing activities during one month incubation. The amount of the increases varied between the soils. Application of rice straw brought about the significant increases during incubation time. Compost contained the largest amount of available-N among the C-sources, and thereby brought the smallest increase in all soils. The cumulative fixed nitrogen with application of rice straw at 30 days incubation in the three experimented soils were highest in Hwadong clay soil, i.e, $2.2mg^N/100g$, intermediate in Ratchaburi soil $0.85mg^N$, and least in Konosu soil $0.80mg^N/100g$. On the other hand, nitrogen fixing heterotrophic, bacteria, such as Clostridia, aerobes and anaerobes, were remarkably increased by application of rice straw while Azotobacter and Beijerinkia were not. The cumulative fixed nitrogen was more pronounced in the clay soil than in the coarse loamy soil. More pronounced nitrogen fixing activities in light condition(heterotrophic + photosynthetic) than that in dark(heterotrophic) condition have been observed both in the coarse loamy and clay soils. The nitrogen fixing ability of photosynthetic microbes in paddy soil is probably higher in coarse loamy soil than in clay soil.

한국(韓國), 일본(日本) 및 태국(泰國)의 논토양(土壤)을 홍시(洪試)하여 수종유기물(數種有機物)을 첨가(添加)했을 때 지양성(地養性) 질소고정력(窒素固定力)에 미치는 영향(影響)을 알고져 실내시험(室內試驗)한 결과(結果)를 요약(要約)하면 다음과 같다. 1. 유기물(有機物) 첨가(添加)에 의(依)한 타양성(他養性) 질소고정(窒素固定) 미생물(微生物)의 질소고정력(窒素固定力)은 Glucose > 볏짚 > 퇴비(堆肥)의 순으로 높고 3개토양간(個土壤間)에 현저(顯著)한 차이(差異)가 있었다. 2. 탄소(炭素) 1g당(當) 질소고정력(窒素固定力)은 토양(土壤)에 따라 상이(相異)하나 담수(湛水)30일간(日間) 췌동(萃東)(한국(韓國)) 2.20mg, 홍소(鴻巢)(일본(日本)) 0.80mg, 그리고 Ratchaburi 토양(土壤)(태국(泰國))이 0.85mg/100g이였다. 3. 논토양(土壤)의 질소고정력(窒素固定力)은 근래(近來) 수년간(數年間) 유기물(有機物) 및 화학비료(化學肥料)를 전혀 시용(施用)하지 않은 토양(土壤)(Ratchabur) 및 화학비료(化學肥料)만을 시용(施用)한 토양(土壤)(홍소(鴻巢))에 비(比)하여 볏짚을 운용(運用)한 토양(土壤)(췌동통(萃東統))에서 현저(顯著)히 높았음. 4. 논토양(土壤) 질소고정미생물(窒素固定微生物)에 의(依)한 전질소(全窒素) 고정력(固定力)은 사질토양(砂質土壤)보다 식질토양(殖質土壤)에서 높으나 광합성(光合成) 질소고정(窒素固定) 미생물(微生物)에 의(依)한 질소고정력(窒素固定力)은 반대(反對)로 식질(殖質)보다 사질토양(砂質土壤)에서 높았다.