Bacills cereus 포자에 대한 pH, 식염 및 솔빈산 칼륨의 영향

Effcts of pH, sodium chloride and potassium sorbate on the germination of Bacillus cereus spores in cooked rice homogenate

  • 이명숙 (부산동의공업전문대학 식품공업과) ;
  • 최종덕 (부산수산대학 식품공학과) ;
  • 장동석 (부산수산대학 식품공학과)
  • Lee Myeong Sook (Dept. of Food Processing, Dong Eui Techenical Junior College of Pusan) ;
  • Choi Jong Duck (Dept. of Food Science and Technology, National Fisheries University of Pusan) ;
  • Chang Dong Suck (Dept. of Food Science and Technology, National Fisheries University of Pusan)
  • 발행 : 1983.01.01

초록

식중독 원인균으로 알려져 있는 Bacillus cereus 균의 포자를 cooked rice homogenate 배지에 접종하여 pH, 식염과 솔빈산 칼륨의 농도를 변화시키면서 배양할 때 포자의 발아에 미치는 영향을 실험한 결과를 요약하면 다음과 같다. 1. 포자의 발아범위는 pH $4.5{\sim}10.0$이었으며 발육최적 pH는 7.0 부근으로 $32^{\circ}C$에서 배양 5시간만에 $10^7/ml$에 도달하였다. 2. 식염농도 $2\%$일 때 포자의 발아는 가장 활발하였고, $5\%$ 이상에서는 농도와 비례하여 감소되었다. 이에 반하여 포자형성율은 $2\%$에서 제일 낮았고 $10\%$에서 가장 높은 값을 나타내었다. 3. 솔빈산 칼륨의 농도가 증가될수록 포자의 발아율은 감소하였고 포자형성율은 약간 증가하였다.

The effects of pH, sodium chloride and potassium sorbate on the germination of Bacillus cereus spores in the medium of cooked rice homogenate were studied. At the range of pH $4.5{\sim}10.0$, the germination of spores were observed. Germinated spores were reached to the number of $10^7/ml$ within 5 hours at $32^{\circ}C$ under the condition of pH 7.0, which was found as optimum pH of germination. In the range of sodium chloride $2{\sim}10\%$, the maximum growth were exhibited under $2\%$ concentration, while it proportionally decreased under the salinity condition higher than $5\%$. The growth of Bacillus cereus were inversely related to the concentration of potassium sorbate within the range of $0{\sim}0.2\%$. Maximum sporulation ratio was observed under the culturing condition: $10\%$ NaCl and $0.2\%$ potassium sorbate in the medium of cooked rice homogenate.

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