Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Isolation of Infectious Pancreatic Necrosis Virus from Goldfish (Carassius auratus) and Chum Salmon (Oncorhynchus keta) in Korea.

금붕어(Carassius auratus)와 연어(Oncorhynchus keta)에서 췌장괴저 바이러스(IPNV)의 분리에 대하여

  • Hah, Youg-Chil (Dept. of Microbiology, Seoul National University) ;
  • Hong, Soon-Woo (Dept. of Microbiology, Seoul National University) ;
  • Kim, Mi-Hee (Dept. of Microbiology, Seoul National University) ;
  • Fryer, J.L. (Dept. of Microbiology, Oregon State University) ;
  • Winton, J.R. (Dept. of Microbiology, Oregon State University)
  • Published : 1984.01.01
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Abstract

Two viruses were isolated from kidney and spleen tissues of goldfish(Carasius auratus) and the ovarian fluid of chum salmon(Oncorhynchus keta). Both viruses replicated and produced cytopathic effect in EPC, CHSE-214, and CHH-1 cell lines at $15^{\circ}C$. The isolates were resistant to pH 3 and choloroform. Antiserum to infections pancreatic necrosis virus(IPNV) serotype VR 299 neutralized the infectivity of both of the isolates. Electron microscopy showed that the particles had typical IPNV particle morphology with average diameters of 55nm, This paper describes the first isolation of viruses infecting cultured fish in Korea.

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