Microbiology and Biotechnology Letters (한국미생물·생명공학회지)
- Volume 13 Issue 4
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- Pages.345-348
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- 1985
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Transformation of Bacillus subtilis Protoplast by Recombinant Plasmid DNA
재조합 Plasmid DNA에 의한 Bacillus subtilis의 형질전환
- Kim, Sang-Dal (Department of Molecular and Medical Microbiology, University of Arizona College of Medicine) ;
- John Spizizen (Department of Molecular and Medical Microbiology, University of Arizona College of Medicine)
- Published : 1985.12.01
Abstract
Recombinant chimeric plasmid constructed with Xba I digested pUBl10 and -pE194 was transformed by polyethylene glycol induced protoplast transformation system into Bacillus subtilis BR 151 on the mannitol regeneration media, and two genes of antibiotics resistance were expressed simultaneously in the transfromant. Transformation frequency of the recombinant plasmid was 6.5
Mannitol hypertonic regeneration media를 사용하는 PEG-induced protoplast transformation system을 이용해서 pUB110과 pE194의 recombinant plasmid로 B. subtilis BR151을 transformation 시킴으로써 두 plasmid에서 유래되는 각각의 Neo
Keywords