Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Studies on immobilization and application of beta-galactosidase II. Preparation and properties of the immobilized enzyme from Aspergillus niger CAD 1

beta-Galaetosidase의 고정화 및 응용에 관한 연구 제2보: Aspergillus niger CAD 1의 효소 교정화 조건 및 고정화 효소의 성질

  • Lee, Yong-Kyu (Department of Dairy Science, College of Agriculture, Chonnam National University) ;
  • Cuun, Soon-Bae (Department of Biology, College of Natural Sciences, Chonnam National University) ;
  • Choi, Won-Ki (Department of Chemistry, College Natural Sciences, Chonnam National University) ;
  • Chung, Ki-Chul (Department of Dairy Science, College of Agriculture, Chonnam National University) ;
  • Bae, Suk (Department of Biology, College of Natural Sciences, Chonnam National University) ;
  • Kim, Kwan-Chun (Department of Biology, College of Natural Sciences, Chonnam National University)
  • 이용규 (전남대학교 농과대학 낙농학과) ;
  • 전순배 (전남대학교 자연과학대학 생물학과) ;
  • 최원기 (전남대학교 자연과학대학 화학과) ;
  • 정기철 (전남대학교 농과대학 낙농학과) ;
  • 배석 (전남대학교 자연과학대학 생물학과) ;
  • 김관천 (전남대학교 자연과학대학 생물학과)
  • Published : 1986.12.30
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Abstract

The beta-galactosidase of Aspergillus niger CAD 1 described in previous report was immobilized to cellulose triacetate. The optimal conditions for immobilization of the enzyme were obtained when 13%(v/v) of the soluble enzyme (349 unit/ml) was entrapped in fiber prepared from 10%(w/v) cellulose triacetate in methylene chloride. The optimal temperature and pH for the activity of the immobilized enzyme were $45^{\circ}C$ and 4.5, respectively, which stowed the same values as those of the soluble enzyme. It was found that Km of the immobilized enzyme for lacotse exhibited high value compared with that of the soluble enzyme. The immobilized enzyme showed good storage stability, reusability, and also hydrolysis of lactose when introduced into skim milk and acidic whey.

제1보에서 보고한 Aspergillus niger CAD 1의 beta-galactosidase를 cellulose triacetate를 담체로 하여 고정화조건을 검토하였던바, 13%(v/v)의 정제된 효소와 10%(w/v)의 cellulose triacetate를 30분간, 150stroke/min으로 진탕하면서 포괄시킨 고정화 효소의 활성이 가장 좋았다. 고정화 효소의 최적온도는 $45^{\circ}C$, 최적 pH는 4.5로서수용성 효소의 것과 같았다. 기질로서 ONPG와 유당에 대한 Km값은 각각 $8.3{\timess}10^{-3}$과 $117.0{\times}10^3M$, Vmax값은 각각 $30.3{\times}10^3M$과 $5.0{\times}10^3M$이였다. 본 고정화 효소는 $4^{\circ}C$에서 90일간 저장시에 90%, $45^{\circ}C$에서 5회 사용후에 70%의 잔존활성을 나타냈으며 유당분해율도 좋았다.

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