Korean Journal of Microbiology (미생물학회지)
- Volume 24 Issue 1
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- Pages.18-23
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- 1986
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
Characterization of the Restriction Endonuclease Bki I from Brevibacterium divaricatum
Brevibacterium divaricatum의 제한효소 Bdi I의 특성
Abstract
A new type II restriction endonuclease, Bdi I, has been isolated from Brenibacterium divaricatum FERM 5948 by procedures of ammonium sulfate fractionation, DEAE-cellulose chromatography and heparin agarose chromatography. The purified Bdi I restriction endonudlease had the same cleavage patterns of Cla I whose recognition sequence is 5' ATCGAT 3'. From the result that
Breνibacterium divaricatum FERM 5948 균주로 부터 Bdi I 제한효소를 ammonium sulfate분획, DEAE-cellulose chroma tograph 그리고 heparin agarose chromatograph 방법을 거쳐 부분정제하여 그 효소적 특성을 관찰하였다. 분리한 Bdi I 제한효소는 pBR 322와
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