Characteristics of MCPA plasmid isolated from pseudomonas sp.

Pseudomonas에서 분리한 MCPA 플라스미드의 특성

  • 이영록 (고려대학교 이과대학 생물학과) ;
  • 최대성 (고려대학교 이과대학 생물학과) ;
  • 은성호 (고려대학교 이과대학 생물학과) ;
  • 박영두 (고려대학교 이과대학 생물학과)
  • Published : 1986.12.01

Abstract

From the lysates of the 7 selected strains of Pseulomonas utilizing 2-methyl-4-chlorophenoxyactate as a sole source of carbon and energy, several MCPA plasmids, which encodes genes for the degradation of 2-methyl-4-chlorophenoxyacetate, were isolated, and measured their molecular weight as well as genetic characters such as resistance to antibiotics and degradative ability of other chlorinated herbicides. Transmissibility of the MCPA plasmids, pKU1, pKU15, and pKU17 was tested by conjugation or transformation and the restriction pattern of pKU15 for Pvu II, Hind III, EcoR I, Xho I, Bgl II, and Ava II was analyzed.

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