Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
권호 표지

A new restriction endonuclease from xanthomonas citri

새로운 type II 제한효소 xci I의 분리

  • Whang, Y. (Department of Biological Science and Engineering Korea Advanced Institute of Science and Technology) ;
  • Chae, K. S. (Department of Biological Science and Engineering Korea Advanced Institute of Science and Technology) ;
  • Jang, W. H. (Department of Biological Science and Engineering Korea Advanced Institute of Science and Technology) ;
  • Kim, K. T. (Department of Biological Science and Engineering Korea Advanced Institute of Science and Technology) ;
  • Yoo, O. J. (Department of Biological Science and Engineering Korea Advanced Institute of Science and Technology)
  • 황영 (한국과학기술원 생물공학과) ;
  • 채건상 (한국과학기술원 생물공학과) ;
  • 장원희 (한국과학기술원 생물공학과) ;
  • 김기태 (한국과학기술원 생물공학과) ;
  • 유욱준 (한국과학기술원 생물공학과)
  • Published : 1986.12.01
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Abstract

The isolation and charateriation of a type II restriction endonuclease from Xanthomonas citri IFO 3835 were described. This enzyme (Xci I endonuclease) is an isoschizomer of Sal I endonuclease recognizing 5'-GTCGAC-3' and cleaving at the site indicated by the arrow. Unlike Sal I endonuclease, Xci I endonuclease requries a NaCl concentration of 50mM for its maximum activity.

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