Synthesis of $\textrm{N}_{G}$-Mon $o^{14}\textrm{C}$-methyl］-L-arginine

$\textrm{N}_{G}$-Mon $o^{14}\textrm{C}$-methyl］-L-arginine의 합성

Radiochemical synthesis of $N^{G}$-mono［$^{14}$ C-methyl］-L-arginine is described. The compound was synthesized from radio-active mono［$^{14}$ C］-methylamine as easily and purified by strong cation-exchange resin (NH form) liquid chromatography using a gradient of ammonium hydroxide, and crystallized as flavianate. The free amino acid was successfully prepared by strirring its flavianate and strong anion-exchange resin (OH- form), which could remove the flavianic acid from its salt in water below room temperature. Purity of the compound was tested by thin-layer chromatography, thin-layer electro-phoresis, and scintillation spectrometry.y.

$N^{G}$ -Mono［$^{14}$ C-Methyl］-L-arginine을 방사선화학적 방법으로 mono ［$^{14}$ C］-Methylamine 으로부터 합성한후 양이온 교환수지에 흡착시킨 다음 암모니아수로 용출시켜 정제하였으며 flavianic acid를 사용하여 결정상태로 얻었다. 한편 flavianate와 음이온 교환수지 를 함께 실온 이하의 온도에서 교반혼합함으로서 유리 상태의 amino acid를 쉽게 만들수 있으며 박층크로마토그라피, 박층전기영동 및 섬광분광분석법으로 순도를 조사하였다.