한국균학회지 (The Korean Journal of Mycology)
- 제15권2호
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- Pages.80-86
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- 1987
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- 0253-651X(pISSN)
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- 2383-5249(eISSN)
섬유소 분해균 Aspergillus sp. HB 1의 세포융합에 관한 연구
Cell Fusion of Cellulolytic Fungi, Aspergillus sp. HB1
- Kim, Joo-Ho (Department of Genetic Engineering, College of Natural Sciences, Hanyang University) ;
- Chang, Sung-Yeoul (Department of Biology, College of Natural Sciences, Hanyang University) ;
- Choi, Yong-Keel (Department of Biology, College of Natural Sciences, Hanyang University)
- 발행 : 1987.06.30
초록
자연계에서 섬유소분해능이 우수한 균을 분리하였고 섬유소분해능을 더욱 증진시키기 위한 기초단계로서 그 분리균주의 원형질체 생성과 융합의 최적조건을 조사하였다. 토양에서 채취한 시료로부터 섬유소분해능력이 우수한 Aspergillus sp., Penicillium sp., Trichoderma sp., 3균주를 분리 동정하였고 산업적으로 유용한 균주이며 cellulase activity가 있는 Aspergillus niger를 비교균주로 선택하여 각각의 효소활성도를 비교 조사한 결과, Aspergillus sp.가 가장 좋은 효소활성도를 보였으며 그 다음 Penicillium sp., Trichoderma sp., 그리고 Aspergillus niger 순이었다. 효소활성도가 가장 좋은 Aspergillus sp.에 자외선
The author isolated high cellulolytic fungi from natural sources and determined optimal condition of protoplast formation and fusion as fundamental step for improvement of the isolated it's cellulolytic ability. Three different cellulolytic fungi, such as Aspergillus sp., Penicillium sp. and Trichoderma sp., were isolated from soil. Their cellulolytic activities were compared with that of Aspergillus niger which was useful industrially and had cellulase activity. It was Aspergillus sp. that showed the highest activity of all these four fungi. And then it was followed by Penicillium sp., Trichoderma sp., and Aspergillus niger in order. An auxotrophic mutant of Aspergillus sp. was obtained by UV mutagenesis method. Having try to produce protoplast from mycelia, the author found that