The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Studies on the Development of Photoreceptor in the Nonchromatophore Organisms (II) - Effects of organic compound and metal ion influx of Light-Induced Mitochondrial ATPase in the Lentinus edodes(Berk.) Sing -

무흡광색소 식물의 감광수용체 개발 연구(II) - 표고버섯의 광감응성 mitochondrial ATPase의 유기물 및 금속이온 유입 효과 -

  • Min, Tae-Jin (Department of Chemistry, College of Sciences, Dongguk University) ;
  • Cho, Suck-Woo (Department of Chemistry, College of Sciences, Dongguk University) ;
  • Kim, Young-Soon (Department of Chemisty, College of Sciences, Dongguk University) ;
  • Kim, Jae-Woong (Yuhan Technical College) ;
  • Mheen, Tae-Ick (Korea Advanced Institute of Science and Technology)
  • Published : 1987.12.30
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Abstract

Effects Of organic compound, photosensitizer and $K^+$ ion influx. On the light-induced ATPase of mitochondria in L. edodes purified by linear sucrose density gradient centrifugation were studied. The mitochondrial ATPase activity was investigated by various wavelength illumination at dark state. The mitochondrial ATPase was activated 139% and 128% by 10m mol dithiothreitol and 0.1m mol quinacrine, respectively. This enzyme also was activated 36% by 0.1m mol phenazine methosulfate as photosensitizer. But, 100 mg oligomycin and 1m mol phlorizin inhibited activity of enzyme to 48% and 45%, respectively. Its optimum wavelength was 690 nm on the effect of $K^+$ ion influx, its optimum pH and temperature were found to be 7.2 and $55^{\circ}C$.

표고버섯의 미토콘드리아는 설탕밀도 선형기울기 원심분리법으로 정제하여, 광감응성 mitochondrial ATPase의 유기물 효과, 광증감제 효과 및 파장 변화에 따른 $K^+$ 이온의 유입 효과를 실험하였다. 1. 이 효소는 10m mol dithiothreitol 및 0.1m mol quinacrine에 의하여 각각 139% 및 128%의 활성도를 증가시켰다. 2. $100\;{\mu}g$의 oligomycin과 1m mol phlorizin은 이 효소의 활성을 각각 48% 및 45% 억제시켰다. 3. 광증감제인 0.1m mol phenazine methosulfate는 이 효소의 활성도를 36% 촉진시켰다. 4. $K^+$ 이온 유입 효과의 최적 파장은 690nm였고, 이때의 최적 pH 및 최적 온도는 각각 7.2 및 $55^{\circ}C$였다.

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