Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Partial Purification and Characteristics of Amylases from Herpetosiphon geysericola

Herpetosiphon geysericola 균주의 Amylase 부분정제 및 특성

  • Jun, Yeong-Soo (Department of Food and Nutrition, Pusan National University) ;
  • Hong, Yong-Ki (Department of Biological Science and Technology, National Fisheries University of Pusan) ;
  • Seu, Jung-Hwn (Department of Microbiology, Kyungpook National University)
  • 전영수 (부산대학교 식품영양학과) ;
  • 홍용기 (부산수산대학 생물공학과) ;
  • 서정훈 (경북대학교 미생물학과)
  • Published : 1987.05.30
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Abstract

Extracellular ${\alpha}-amylase$, ${\beta}-amylase$ and glucoamylase produced by a thermophilic and cellulolytic bacterium, Herpetosiphon geysericola CUM 317, were partially purified by salting out with ammonium sulfate and by chromatography on a DEAE-cellulose column and on a CM-cellulose column. The Km values of ${\alpha}-amylase$, ${\beta}-amylase$ and glucoamylase for potato starch were $2.31mg/m{\ell}$, $7.69mg/m{\ell}$, and $8.33mg/m{\ell}$. The molecular weights of ${\alpha}-amylase$, ${\beta}-amylase$ and glucoamylase were calculated to be about 84000 dalton, 76000 dalton and 80000 dalton, respectively.

고온성 cellulose 분해이용세균인 Herpetosiphon geysericola CUM 317 균주가 생성하는 ${\alpha}-amylase$, ${\beta}-amylase$ 및 glucoamylase를 황산암모늄 염석, DEAE-cellulose chromatography, CM-cellulose chromatography 방법으로 각각 부분정제하였다. 이들 ${\alpha}-amylase$, ${\beta}-amylase$ 및 glucoamylase의 감자 전분에 대한 Km치는 $2.31mg/m{\ell}$, $7.69mg/m{\ell}$ 및 $8.33mg/m{\ell}$였으며, 각 분자량은 84,000 dalton, 76,000 dalton 및 80,000 dalton의 크기로 나타났다.

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