Determination of Gypsogenin by HPLC Using 2, 4-Dinitrophenylhydrazine as a Pre-labeling Reagent

HPLC에 의한 Gypsogenin-2,4-DNPH의 정량

Gypsogenin was derivatized with 2, 4-dinitrophenylhyclrazine prior to analysis with HPLC. Reversed-phase column (Du pont ODS) was used and the mobile phase was acetonitrile and water (60:40). The effluent was detected at 550nm using an U.V. detector and the retention time was approximately 9.2min. The concentration was quantitated by measuring the area and the detection limit was 0.2$\mu\textrm{g}$.