Relationships between Radiation-induced Prostaglandin E2 and Natural Killer Cell Activity in Mice

방사선조사(放射線照査)에 의한 Prostaglandin E2 및 자연살해세포(自然殺害細胞) 활성도(活性度)의 변화(變化)

  • Kim, Sung-ho (Laboratory of Cancer Pathology, Korea Cancer Center Hospital, Korea Advanced Energy Research Institute)
  • 김성호 (한국에너지연구소 원자력병원 암병리학연구실)
  • Received : 1987.07.24
  • Published : 1987.10.10

Abstract

The number of splenic lymphocyte, serum prostaglandin $E_2$ level and natural killer cell activity were assayed after single whole body irradiation of a sublethal dose of $^{60}Co-{\gamma}$ ray to C57BL/6J mice. With a view to knowing the relationships between radiation induced prostaglandin $E_2$ level and the normal natural killer cell activity after natural killer cell-target cell conjugation, The change of normal natural killer cell activity were measured by administration of prostaglandin $E_2$ containing serum from irradiated mice. The results were summarized as follows; 1. The total number of splenic lymphocyte was significantly decreased by irradiation and the number was not affected by indometacin, prostaglandin synthesis inhibitor, treatment. 2. Serum prostaglandin $E_2$ level was increased in irradiated mice, but indometacin treated mice group showed low level of prostaglandin $E_2$. 3. In the case of irradiated mice, natural killer cell activity was not shown any difference between irradiated group and indometacin combined group. But when natural killer cell-target cell conjugations were exposed to the serum of each group during cytotoxic activity assay, whereas the normal natural killer cell activity was significantly decreased by treatment of serum from irradiated mice, the activity was not changed by treatment of indometacin pretreated mice serum. This result indicated that the prostaglandin $E_2$ induced by the radiation inhibited the post-target binding cytolytic process of natural killer activity.

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