Lyophyllum decastes를 이용(利用)한 볏짚의 발효사료(醱酵飼料)에 관한 연구(硏究) -I. Cellulase 생산조건(生産條件) 및 배양기간(培養期間)의 영향(影響)-

Studies on Improving the nutritive value of Rice straw by Fermentation with Lyophyllum decastes -I. Cultural condition of cellulase production and feeding value under fermentation periods.-­

Lyophyllum decastes의 발효사료 제조를 위한 cellulase 생산은 $30^{\circ}C$, pH 6.0, 수분 $60{\sim}70%$ 범위에서 I5일간 배양하는 것이 좋았다. 부원료로 미강 $30{\sim}40%$와 $(NH_4)_2HOP_4$ 0.72%를 첨가하고 볏짚을 4% alkaline peroxide로 전처리하면 cellulase 생산은 증가하였다. 볏짚에 Lyophyllum decastes를 40일간 발효시켰을 때 lignin은 19.9% 분해되있고 총질소는 대조구의 1. 1%에서 1.6%로 증가되었다. 발효가 진행됨에 따라 볏짚의 in vitro 소화율은 증가하여 35일 발효 후에는 대조구에 비하여 21. 1%증가하였다.

For the preparation of fermented feed with Lyophyllum decastes, optimum cultural conditions for the production of cellulase were $30^{\circ}C$, pH 6.0, 60-70% moisture content and the cultural of 15 days. Among the submaterial added, 30-40% of rice bran and 0.72% of $(NH_4)_2PHO_4$ were effective for the cellulase production and its production increased when rice straw treated with 4% alkaline peroxides. Solid state fermentation of rice straw with Lyophyllum decastes for 40 days removed 19.9% of lignin, and increased the total nitrogen content to 1.6% from 1.1%. As the fermentation proceeded, the in vitro dry matter digestibility of fermented feed was increased and it increased 21.1% after 35 days.