Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Studies on the Optical Resolution of DL-Amino Acids by Aminoacylase Immobilized on Chitosan: Immobilization of Aminoacylase

Chitosan 고정화 Aminoacylase를 이용한 DL-아미노산의 광학적 분할에 관한 연구 : Aminoacylase 의 고정화

  • Lee, Sang-Hyun (Department of Food Science & Technol., Chung-Ang University) ;
  • Lee, Young-Chun (Department of Food Science & Technol., Chung-Ang University)
  • 이상현 (중앙대학교 식품가공학과) ;
  • 이영춘 (중앙대학교 식품가공학과)
  • Published : 1988.08.01
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Abstract

Optimum conditions for immobilization of aminoacylase on chitosan were investigated, and the results are summerized as follows: Optimum conditions for activation of chitosan were pH 6.0, 0.2% of glutaraldehyde, and 120 minutes of reaction time. Enzyme concentration and reaction time for immobilization of aminoacylase on the activated chitosan were 80mg/20ml, and 90 minutes, respectively, and the yield of activity of the immobilized enzyme was 42.6%.

Chitosan을 담체로 이용하여 aminoacylase를 고정화하는 조건을 연구한 결과는 다음과 같다. 담체의 최적 activation조건은 pH 6.0, glutaraldehyde 0.2%, 반응시간 120분이었다. 그리고 activated chitosan에 aminoacylase를 고정화하는데 가장 적당한 효소농도는 80mg/20ml 이었고, 이때 coupling time은 90분이 가장 적절하였다.

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