Pseudomonas putida KU 190에서 분리한 R plasmid pKU 41의 특성

Characterization of R plasmid pKU 41 from pseudomonas putida KU190

  • 이윤희 (고려대학교 이과대학 생물학과) ;
  • 주미자 (목원대학교 미생물학과) ;
  • 이영록 (고려대학교 이과대학 생물학과)
  • 발행 : 1988.03.01

초록

The location of R-determinants, $Ap^{r}$ and $Tc^{r}$, and replication origin in pKU41 determined using the construction of miniplasmid by the BamHI and the HindIII restriction fragment from pKU41 and the cloning of the restriction fragments from pKU41 into pSY343. The gene encoding resistance to ampicillin (Ap) as well as replication origin in pKU41 were located on the region overlapping BamHI B fragment and HindIII A fragment. The gene encoding resistance to tetracycline (Tc) was located on the region of the HindIII C fragment, which was cleaved by BamHI as well.

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