Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Isolation of Nif$^{-10}$ -mutants through transposon mutagenesis in enterobacter agglomerans 339

Enterobacter agglomerans 339에 있어서 transposon umtagenesis를 통한 Nif$^{-10}$ -mutants 분리 동정

  • 민병환 (경희대학교 문리과대학 생물학과) ;
  • 이호자 (경희대학교 문리과대학 생물학과)
  • Published : 1988.03.01
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Abstract

Three $NIf^{-}$ -mutants were isolated from Enterbacter agglomerans 339 through the transposon umtagenesis using a RP4-mobilising system for its nif-gene characterization. All mutants hadn't acetylene-reduction ability. Then we confirmed that Tn5 was inserted into all conserved nif-plasmids through the Southern Hybridization.

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