Korean Journal of Microbiology (미생물학회지)
- Volume 26 Issue 1
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- Pages.37-43
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- 1988
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
Protoplast fusion between saccharomyces cerevisiae and candida cariosilignicola
Saccharomyces cerevisiae와 candida cariosilignicola사이의 세포융합에 관한 연구
Abstract
This research was focused on investigation of the condition for protoplast formation and regeneration of protoplast fusion between Saccharomyces cerevisiae which has fermentation ability and Candida cariosilignicola which can grow at high temperature and utilize methanol. The results obtained were as follows; The highest production was collected in exponential growth phase. Ninety-nine% protoplast formation of C. cariosilignicola was obtained in glycin-NaOH buffer (pH10.0) containing Zymolyase 0.5mg/ml at