Korean Journal of Microbiology (미생물학회지)
- Volume 26 Issue 1
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- Pages.52-59
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- 1988
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
Characterization of L-Galactono-1, 4-lactone Oxidase Purified from Saccharomyces cerevisiae
Saccharomyces cerevisiae에서 분리한 L-Galactono-1, 4-lactone Oxidase의 특성
Abstract
A partially purified preparation of L-galactonolactone oxidase which catalyzes the last step of L-ascorbic acid biosynthesis was obtained from Saccharomyces cerevisiae ATCc 26787. The purification procedures included Triton X-100 treatment, protamine sulfate precipitation, ammonium sulfate precipitation, DEAE-Sepharose CL-6B ion exchange chromatography, Sephadex G-150 gel filtration chromatography, and Phenyl-Sepharose CL-4B hydrophobic interaction chromatography. The optimum temperature for the enzyme activity was about