미생물학회지 (Korean Journal of Microbiology)
- 제26권2호
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- Pages.129-136
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- 1988
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
탄수화물과 황산암모늄이 Pseudomonas diminuta의 리그닌 분해에 미치는 영향
Effects of Some Carbohydrates and Ammonium Sulfate on Lignin Degradation by Pseudomonas diminuta
초록
To investigate the influence of cosubstrate supplement and ammonium sulfate on lignin degradation by Pseudomonas diminuta KM-4-2, isolated in the laboratory, the strain was cultured on the lignin media which contained lignin as a source of carbon and the culture filtrate was analyzed by Sephadex G-75 column chromatography. It was found that polymerization was not appeared unlike wood-rot fungi. When the carbohydrates were added, the peak of lignin at 280nm by UV scanning spectra of the filtrate, was significantly increased. In order to determine the effect of ammonium sulfate on the ligninolytic activity, the isolated strain was incubated in the media containing 0.1%, 0.25% and 0.5% of nitrogen concentration in the Warburg flask and the rate of oxygen uptake was esitmated by Warbuge Respirometer. As a result, the activity was maximum at 0.1% of nitrogen concentration and thereafter decreased in parallel with nitrogen concentration.