미생물학회지 (Korean Journal of Microbiology)
- 제26권4호
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- Pages.305-311
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- 1988
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
Transposon Tn5를 이용한 Slow growing Rhizobium japonicum의 돌연변이 유도
Mutagenesis of Slow Growing Rhizobium japonicum by Transposon Tn5
- Kim, Sung-Hoon (Genetic engineering Center, KAIST) ;
- Rhee, Yoon (Genetic engineering Center, KAIST) ;
- Sun, Dae-Kyu (Genetic engineering Center, KAIST) ;
- Yoo, Ick-Dong (Genetic engineering Center, KAIST)
- 발행 : 1988.12.01
초록
Slow growing R. japonicum R-l68 균주로부터 spectinomycin 내성 균주를 선발하고 이 Rhizobium내에 Tn-5를 도입시키기 위하여 Tn5가 함유된 E. coli WA 803/pGS9과의 conjugation을 통한 transposon mutagenesis를 실시하였다. 이때 C conjugation을 통한 Tn5 전이 빈도는
The spectinomycin resistant strain of slow growing R. japonicum R-168 was selected to be participated in conjugation with E. coli WA803/pGS9. Tn5 was introduced from suicide vector pGS9 into R. japonicum R-168