Susceptibility of Saccharomyces cerevisiae D-71 and Zygosaccharomyces rouxii SR-S to Zymolyase-20T

Zymolyase-20T에 대한 Saccharomyces cerevisiae D-71과 Zygosaccharomyces rouxii SR-S의 감수성

Susceptibility of a thermophilic strain (D-71) of Saccharomyces cerevisiae and an osmotolerant strain (SR-S) of Zygosaccharomyces rouxii to Zymolyase-20T were studied in various renditions. Content of glucan and mannan in cell wall of Saocharomyces cerevisiae D-71 were 14.5% and 14.8%, and Zygosaccharomyces rouxii SR-S were 24.0% and 19.0%, respectively. Susceptibility of Saccharomyces cerevisiae D-71 cultured in Wickerham synthetic medium containing 0.5% of methionine and 0.1% of glucose to Zymolyase-20T was 66%, and $K_2$HPO$_4$ and aminobenzoic acid were greatly effective to susceptibility. Susceptibility of Zygosaccharomyces rouxii SR-S cultured in Wickerhnin synthetic medium containing 0.5% of peptone, 0.15% of methionine and 0.l% of glucose to Zymolyase-20T was 80%, and KI and pyridoxine were greatly effective to susceptibility. Susceptibility of Saccharomyces cerevisiae D-71 stationary cultured in YMPG medium at $25^{\circ}C$ for 12 hours was 16o1e and Zygosaccharomyces rouxii SR-S stationary cultured in YMPG medium at $25^{\circ}C$ for 30 hours was 82%.

고온 발효성인 Saccharomyces cerevisiae D-71과 내삼투압성인 Zygosaccharomyces rouxii SR-S를 여러 조건에서 배양하여 Zymolyase-20T에 대한 감수성을 조사하였다. Sacch. cerevisiae D-71의 glucan과 mannan 함량은 각각 l4.5%와 14.8% 이었고 Zygosacch. rouxii SR-S는 24.0%와 19.0% 이었다. 0.5% methionine 과 0,1% glucose를 함유한 wickerham 합성배지에 Sacch. cerevisiae D-71를 배양하여 얻은 세포의 Zymolyase-20T에 대한 감수성은 66% 이었고 $K_2$HPO$_4$와 aminobenzoic acid를 결핍시켰을 때 감수성이 가장 낮았다. 또한 0.5% peptone과 o.15% methionine 및 0.1% glucose를 함유한 wickerham 합성배지에 Zygosacch. rouxii SR-S를 배양하여 얻은 세포의 감수성은 80% 이었고 KI와 pyridoxine을 결핍시켰을 때 감수성이 가장 낮았다. $25^{\circ}C$에서 YMPG 배지에 Sacch. cerevisiae D-71를 접종하여 12시간 정치배양한 세포의 Zymolyase-20T에 대한 감수성은 76% 이었고 Zygosacch. reuxii SR-S를 같은 조건으로 30시간 배양한 세포의 감수성은 82%로서 wickerham 합성배지에서의 감수성보다 더 좋았다.