연어알에서 분리한 Carotenoprotein의 구조적 특성

Subunits and Composition of Carotenoprotein from Salmo Salar Eggs

  • Jae-Woong Kim (Department of Food and Nutrition, Yuhan Technical college) ;
  • Tae-Jin Min (Department of Chemistry, Dongguk University) ;
  • Tae-Young Lee (Department of Chemistry, College of Education, Seoul National University)
  • 발행 : 1988.08.20

초록

연어알(Salmo Salar)에서 carotenoprotein을 CM-세룰로스, 50% 황산암모늄 침전, DEAE-세룰로스 및 세파덱스 G-75젤을 사용하여 충분히 정제하였다. 인산염 완충용액(pH 7.0)에서 자외선 흡수극대는 ${\lambda}_{max}$ 409, 540 그리고 580nm이었다. 세파덱스 G-200젤 여과법으로 측정된 분자량은 50, 200rhk 26,000달톤으로 heterogeneous한 두가지 형태였다. 전자는 적자색으로 SDS-PAG 전기영동 결과 분자량이 12,500달톤인 4개의 동일한 subunit로 구성되었고, 상당량의 지질과 만노오스, 갈락토스와 글루코사민을 함유하고 있음을 밝혔다. carotenoprotein의 색소를 추출하여 tlc로 정제한 다음 분배비 시험, 요오드 시험, 에폭시 그룹 시험, 알릴릭 시험, 환원 시험 그리고 아세틸화 시험등의 유기반응과 uv/vis, ir 및 nmr 데이타를 얻어 구조를 결정한 결과 carotenoid는 astaxanthin ester임을 확인하였으며 그 ester는 스테아르산(47.9%)과 팔미트산 (21.4%)이 대부분이었다.

Carotenoprotein from Salmo Salar eggs was purified and characterized by CM-cellulose, 50% $(NH_4)_2SO_4$, DEAE-cellulose and sephadex G-75 column. The chromoprotein had a spectrum with ${\lambda}_{max}$ 409, 540 and 580nm in p-buffer (pH 7.0) at initial step. Molecular weights by sephadex G-200 gel filtration were 50, 200 and 26,000 daltons. SDS-PAGE analysis showed a structure with four identical subunits (12,500 daltons). Its sample retained a small amount of carbohydrates and lipids. Amino acids were analyzed, and mannose, galactose and glucosamine also were identified. Carotenoid extacted with acetone was found to be astaxanthin ester by partition test, epoxy test, iodine test, allylic test, reduction, acetylation, uv/vis, ir and nmr datas. Stearate (47.9%) and palmitate (21.4%) were predominant fatty acids in the astaxanthin ester.

키워드

참고문헌

  1. Arch. Dermatol v.114 D.R. Bickers;L.K. Demar;V.A. DeLeo
  2. Biochem. Biophys. Res. Comm. v.98 J.E. Packer;J.S. Mahood;V.O. Mora-arellano;T.F. Slater;R.L. Willson;B.S. Wolfenden
  3. Cancer Inst. v.69 N.I. Krinsky;S.M. Deneke;J. Natl
  4. Pure & Appl. Chem. v.51 N.I. Krinsky
  5. Am. J. Med. v.60 V. A. DeLeo;M. Poh-Fitzpatrick;M. Mathews-Roth;L. C. Harber
  6. J. Am. Med. Assoc. v.228 M.M. Mathews-Roth;M.A. Pathak;T.B. Fitzpatrick;L.C. Harber;E.H. Kass
  7. J. Am. Med. Assoc. v.236 E.L. Dubois;C. Patterson
  8. Arch. Dermatol. v.113 M.M. Mathews-Roht;M.A. Pathak;T.B. Fitzpatrick;L.H. Harber
  9. Nature v.290 R. Peto;R. Doll;J.D. Buckley;M.B. Sporn
  10. Cancer Res. v.46 I. Fabian;S. Shvartzmayer
  11. Cancer Res. v.46 H.L. Gensler;D.A. Sim;G.T. Bowden
  12. Comp. Biochem. Physiol. v.22 P.F. Zagalsky;D.F. Cheesman;H.J. Ceccaldi
  13. Comp. Biochem. Physiol. v.34 P.F. Zagalsky;H.J. Ceccaldi;R. Daumas
  14. Pure & Appl. Chem. v.47 P.F. Zagalsky
  15. Biochim. Biophys. Acta v.530 A. Elgsaeter;J.D. Tauber;S. Liaaen-Jensen
  16. Nature v.279 M.L. Mackenthum;R.D. Tom;T.A. Moore
  17. Biochim. Biophys. Acta v.576 V.R. Salares;N.M. Young;H.J. Bernstein;P.R. Carey
  18. Biochim. Biophys. Acta v.453 R. Powls;G. Britton
  19. J. Kor. Chem. Soc. v.29 J.W. Kim;T.J. Min
  20. J. Biol. Chem. v.193 O.H. Lowry;N.J. Rosebrough;A.L. Farr;R.J. Randall
  21. Methods of Biochem. Anal. v.XV D.S. Mclaren;W.W.C. Read;Z.L. Awdeh;M. Tchalian
  22. Febs. letter v.58 B. Lugtenberg;J. Meijers;R. Peters;P.V.A. Hoek;L.V. Alphen
  23. Plant Physiol. v.62 D.M. Peterson
  24. Anal. Biochem. v.19 J. Ludowieg;J.D. Benmaman
  25. Comp. Biochem. Physiol. v.45B A. Khare;G.P. Moss;B.C.L. Weedon;A.D. Matthews
  26. Comp. Biochem. Physiol. v.69B B. Renstrom;G. Borch;S. Liaaen-Jensen
  27. Hydrobiologia v.46 B. Czeczuga
  28. Hydrobiologia v.64 B. Czeczuga
  29. Bull. Japan. Soc. Fish v.47 no.3 T. Matsuno;K. Hiraoka;T. Maoka
  30. Chemistry and Biochemistry of Plant Pigments B.H. Davis
  31. Comp. Biochem. Physiol. v.21 H.J. Cecccaldi;P.F. Zagalsky
  32. Carotenoid chem. & Biochem. G. Britton;G. M. Armit;S. Y. M. Lau;A. K. Patel;C. C. Shone
  33. Comp. Biochem. Physiol. v.17 D.F. Cheesman;J. Prebble
  34. Dissertation for ar Celebation of 61 st. Birthday T.Y. Lee
  35. Helv. Chim. Acta. v.64 K. Schiedt;F.J. Leuenberger;M. Vecchi