Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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MEASUREMENT OF SYNTHESIS RATE OF LONG-CHAIN ACYL-COENZYME A ESTER IN BOVINE LIVER BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

  • Mitsuhashi, T. (Chugoku National Agricultural Experiment Station, Livestock Division) ;
  • Mitsumoto, M. (Chugoku National Agricultural Experiment Station, Livestock Division) ;
  • Yamashita, Y. (Hokuriku National Agricultural Experiment Station) ;
  • Ozawa, S. (Chugoku National Agricultural Experiment Station, Livestock Division)
  • Received : 1988.05.06
  • Accepted : 1988.09.07
  • Published : 1988.06.01
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Abstract

A high performance liquid chromatographic procedure is described for the direct determination of the picomole amount of palmitoyl-Coenzyme A and stearoyl-Coenzyme A, using a stainless steel column packed with C-18 derivatized porous silica ($5{\mu}m$), an isocratic elution with a mixture of 33 mM $KH_2PO_4$/acetonitrile as a mobile phase and a UV detector. The long-chain acyl-Coenzyme A esters were determined in incubated microsomal fractions of a bovine liver to demonstrate the utility of this method for monitoring acyl-CoA synthesis in biological samples. The reaction rate of palmitate was higher than that of stearate. After a 60 minute incubation period, the generated amount of palmitoyl-Coenzyme A and stearoyl-Coenzyme A were approximately 70 and 20 n mol/mg micresomal protein, respectively. The advantage of this method are in that no decomposition of the CoA esters is involved, while the constituent molecular species is detected.

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