Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Studies on enzyme immunoassay for determining progesterone of bovine plasma and its clinical application I. Optimizing double antibody for progesterone in enzyme immunoassay

Enzyme immunoassay(EIA)에 의한 소의 혈중 progesterone 측정과 이의 응용에 관한 연구 I. 이항체(二項體)의 최적조건에 관한 연구

  • Kang, Chung-boo (Department of Veterinary Medicine, College of Agriculture, Gyeongsang National University) ;
  • Shin, Jong-uk (Department of Veterinary Medicine, College of Agriculture, Gyeongsang National University) ;
  • Choe, Sang-yong (Department of Veterinary Medicine, College of Agriculture, Gyeongsang National University)
  • 강정부 (경상대학교 농과대학 수의학과) ;
  • 신종욱 (경상대학교 농과대학 수의학과) ;
  • 최상용 (경상대학교 농과대학 수의학과)
  • Received : 1988.07.29
  • Published : 1988.10.15
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Abstract

This experiment was carried out to determine the progesterone concentration of bovine plasma by liquid phase double antibody enzyme immunoassay. The optimum conditions of assay-system, double (first and second) antibody and carrier (normal rabbit serum) were investigated. The optimum dilution rate of first antibody, second antibody and normal rabbit serum was $10{\times}10^3$ to $15{\times}10^3$, 20 and $1{\times}10^3$ times, respectively.

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Acknowledgement

Supported by : 한국과학재단