The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Cloning and Expression of Leu 2 Gene (${\beta}-isopropylmalate$ dehydrogenase) from the Basidiomycete Flammulina velutipes in E. coli

팽나무버섯 균사체에서 ${\beta}-isopropylmalate$ dehydrogenase(Leu 2) gene 의 cloning 및 E. coli에서 발현

  • Byun, Myung-Ok (Applied Mycology and Mushroom Division, Agricultural Sciences Institute, RDA) ;
  • Yoo, Young-Bok (Applied Mycology and Mushroom Division, Agricultural Sciences Institute, RDA) ;
  • Go, Seung-Joo (Applied Mycology and Mushroom Division, Agricultural Sciences Institute, RDA) ;
  • You, Chang-Hyun (Applied Mycology and Mushroom Division, Agricultural Sciences Institute, RDA) ;
  • Cha, Dong-Yul (Applied Mycology and Mushroom Division, Agricultural Sciences Institute, RDA) ;
  • Park, Yong-Hwan (Applied Mycology and Mushroom Division, Agricultural Sciences Institute, RDA)
  • 변명옥 (농촌진흥청, 농업기술연구소 균이과) ;
  • 유영복 (농촌진흥청, 농업기술연구소 균이과) ;
  • 고승주 (농촌진흥청, 농업기술연구소 균이과) ;
  • 유창현 (농촌진흥청, 농업기술연구소 균이과) ;
  • 차동열 (농촌진흥청, 농업기술연구소 균이과) ;
  • 박용환 (농촌진흥청, 농업기술연구소 균이과)
  • Published : 1989.03.30
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Abstract

Gene libraries of DNA from Flammulina velutipes were constructed using Escherichia coli plasmid pBR 322. Leu 2 gene coding ${\beta}-isopropylmalate$ dehydrogenase from F. velutipes was cloned by complementation of leucine requiring mutant of E. coli. The size of inserted DNA fragment of this clone is about 1 Kbp. The fragment has Bam H1 and Ava 1 restriction sites.

팽나무버섯 균사체 DNA를 대장균 프라스미드인 PBR322를 이용하여 gene library를 작성 하였다. 팽나무버섯에서 ${\beta}-isopropyl$ malate dehydrogenase 유전자 클론을 얻었으며 이 클론은 대장균 Leucine 요구성 균주를 complementation 시켰다. 이 클론의 팽나무버섯 DNA 크기는 약 lKb였으며 BamH1과 Ava 1 제한효소 절단부위를 지니고 있었다.

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