The Korean Journal of Mycology (한국균학회지)
- Volume 17 Issue 1
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- Pages.35-38
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- 1989
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- 0253-651X(pISSN)
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- 2383-5249(eISSN)
Cloning and Expression of Leu 2 Gene (${\beta}-isopropylmalate$ dehydrogenase) from the Basidiomycete Flammulina velutipes in E. coli
팽나무버섯 균사체에서 ${\beta}-isopropylmalate$ dehydrogenase(Leu 2) gene 의 cloning 및 E. coli에서 발현
- Byun, Myung-Ok (Applied Mycology and Mushroom Division, Agricultural Sciences Institute, RDA) ;
- Yoo, Young-Bok (Applied Mycology and Mushroom Division, Agricultural Sciences Institute, RDA) ;
- Go, Seung-Joo (Applied Mycology and Mushroom Division, Agricultural Sciences Institute, RDA) ;
- You, Chang-Hyun (Applied Mycology and Mushroom Division, Agricultural Sciences Institute, RDA) ;
- Cha, Dong-Yul (Applied Mycology and Mushroom Division, Agricultural Sciences Institute, RDA) ;
- Park, Yong-Hwan (Applied Mycology and Mushroom Division, Agricultural Sciences Institute, RDA)
- 변명옥 (농촌진흥청, 농업기술연구소 균이과) ;
- 유영복 (농촌진흥청, 농업기술연구소 균이과) ;
- 고승주 (농촌진흥청, 농업기술연구소 균이과) ;
- 유창현 (농촌진흥청, 농업기술연구소 균이과) ;
- 차동열 (농촌진흥청, 농업기술연구소 균이과) ;
- 박용환 (농촌진흥청, 농업기술연구소 균이과)
- Published : 1989.03.30
Abstract
Gene libraries of DNA from Flammulina velutipes were constructed using Escherichia coli plasmid pBR 322. Leu 2 gene coding
팽나무버섯 균사체 DNA를 대장균 프라스미드인 PBR322를 이용하여 gene library를 작성 하였다. 팽나무버섯에서