The Processing Conditions of Extracts from Rapid Fermented Anchovy Sauce

속성멸치간장엑기스분의 가공조건

AS a part of investigation to use anchovy more effectively as food source, this work was undertaken the processing conditions of extracts from rapid fermented anchovy sauce. To prepare rapid fermented products, the chopped anchovy was mixed with 50% water (w/w), and then hydrolyzed by autolysis or addition of koji under different conditions of hydrolysis. The optimal conditions for hydrolysis of chopped anchovy were revealed $50^{\circ}C$, 6 hours, pH 8.0 by autolysis, and ,$50^{\circ}C$, 6 hours, pH 7.0 by addition of 10% koji, respectively. But, pH control was not much beneficial in increasing yield. The effect of soybean protein isolote for improvement of bitter taste was also tested. The reasonable amount of added soybean protein isolate was revealed 5% on the weight basis of the chopped anchovy. The reaction mixture hydrolyzed under optimal conditions were added with 1% onion powder (w/w), 1% garlic powder(w/w) and 1% red pepper powder(w/w) for masking fishy odor, inactivated for 20 min at $100^{\circ}C$, and then centrifuged for 20 min at 4,000 rpm. The supernatant liquor was filtrated and evaporated to 50%(v/v). finally, table salt was added for bateriostatic effect and characteristic taste of rapid fermented products. the reasonable amount of added table salt was reversed 15% on the volume basis of the evaporated liquor. The hydrolysis ratio of product made by addition of water, product made by addition of koji and water, and product made by addition of soybean protein isolate, koji and water hydrolyzed under optimal conditions were 58.4%, 82.1% and 86.2%, respectively.

우리나라 연안에서 일시에 대량으로 어획되고, 영양적으로 우수하나 원료학적 특성 및 가공적성이 좋지 못해 일부는 비식용사료로 이용되고 있는 멸치를 신속하게 대량처리하여 보다 효율적으로 이용하기 위한 방안의 하나로 속성멸치간장엑스분의 제조를 시도하였다. 마쇄한 멸치의 최적가수분해조건은 자가소화구와 코오지첨가구 모두 $50^{\circ}C$에서 최대활성을 나타내었고, 분해시간은 6시간이 가장 적합하였다. pH는 자가소화구의 경우 pH 8.0부근에서 코오지의 첨가구의 경우 pH 7.0이 가장 좋았고, 코오지농도는 마쇄한 멸치에 대해 10%가 가장 적합하였으며, 식염은 가수분해 이후의 공정에서 첨가하는 것이 바람직하였다. 실제 제품화 할 경우 수율증가를 감안하면 pH조절은 필요하지 않으리라 생각된다. 저장성과 독특한 맛을 고려하여 최종공정에서 첨가하는 가장 적합한 식염농도는 농축액에 대하 15%이었고, 쓴맛교정을 위하여 첨가하는 분리대두단백질의 최적조건은 마쇄한 멸치에 대해 5% 이었다. 구명된 최적조건하에서 가수분해시킨 제품(C), (A) 및 (B)의 가수분해율은 각각 58.4%, 32.1%, 86.2% 이었다.