Cultural Condition of Enterobacter agglomerans U-1 for Polysaccharide Production

Enterobacter agglomerans U-1의 다당류 생산을 위한 배양조건

The cultural condition of Enterobacter agglomerans U-1, a polysaccharide producing soil bacterium, was examined. The optimal medium composition was that contains the following components per liter of distilled water; $sucrose(23.75\;g/L),\;peptone(2.06\;g/L),\;yeast\;extract(0.5\;g/L),\;KH_2PO_4(1.0\;g/L)$ and $MgSO_4{\cdot}7H_2O(1.0 g/L)$. The optimum temperature for polysaccharide production was $30^{\circ}C$, where 8.5 g/L of polysaccharide was produced. The apparent viscosity of fermentation broth after 3 days was 240 mPa.s. at $70sec^{-1}$. The product yield and specific productivity were 36% and 142.07 mg/g/h.

점질성 물질을 생산하는 세균인 Enterobacter agglomerans의 다당류 생산조건을 검토하였다. 최적 조건은 배지로서 $sucrose(23.75g/L),\;peptone(2.06g/L),\;yeast\;extract(0.5g/L),\;KH_2PO_4(1.0g/L)$ 및 $MgSO_4{\cdot}7H_2O(1.0g/L)$를 함유하며 배양 pH와 온도는 6.5와 $30^{\circ}C$이었다. 이 조건에서 3일 배양후 8.5g/L의 다당류가 생산되며 점도는 240 mPa.s.를 나타내었다. 이때의 다당류 생산수율 및 다당류 생산 속도는 각각 36%와 142.07 mg/g/h이었다.