Korean Journal of Microbiology (미생물학회지)
- Volume 27 Issue 2
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- Pages.92-97
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- 1989
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
Construction of rhizobium-E. coli shuttle vector using replication and mobilization function of indigenous multicopy plasmid from rhizobium
Rhizobium muliticopy plasmid의 복제 및 이주 기능을 이용한 rhizobium-E. coli shuttle vector 구축
Abstract
the vector, pGUR19, for Rhizobium gene manipulation, was constructed by combining the replication and mobilization function of indigenous multicopy plasmid from Acacia(Robinia pseudoacacia L.) Rhizobia sp86 with E. coli cloning vehicle, pBR322. The vector could be efficiently mobilized by RP4 tra function incorporated into chromosome of E. coli named SM10 and efficiently transferred to various gram negative hosts including Rhizobium and Afrobacterium by transformation. Mobilization frequency of the constructed vector was ranged from