Korean Journal of Microbiology (미생물학회지)
- Volume 27 Issue 3
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- Pages.279-284
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- 1989
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
Isolation and characterization of sodium dodecylbenzenesulfonate(soft type)-degrading bacteria
Sodium dodecylbenzenesulfonate(soft type)를 분해하는 미생물의 분리 및 특성
Abstract
Macroorganisms capable of utilizing sodium dodecylbenzenesulfonate(SDBS, soft type) as a sole carbon source were isolated from nature by using SDBS agar plate technique. Iwolated bacteria were examined primarily for biodegradation ability of SDBS, and followed by testing for resistance to several kinds of metal compounds and antibiotics. Among them(152 strains), one strain showed a excellent SDBS-degrading ability with a resistance to amipicillin and rifampicin was selected. This bacterium was identified as Klebsiella sp. and harbored two plasmids of about 4 and 5 kilobases. SDBS-degrading ability was lost when the plasmids were cured by mitomycin C. It was revealed that the degradation of SDBS was controlled by the plasmid DNA encoding genes. The two plasmids were stably maintained in Escherichia coli C600.
부산시내 주택단지의 하수나 토양에서 LAS 분해능이 우수한 균주를 유일한 탄소원으로서 sodium dodecylbenzenesulfonate(SOBS, soft type)를 포함하는 agar plate를 사용하여 분리히였다 이들 분리균중 2균주가. SOBS를 0.1% 함유한 완전배지에서 30