Properties of Promoters Transferred to the Donor Strain, Alkali-tolerant Bacillus sp. YA-14.

공여 균주인 알카리 내성 Bacillus속에 도입된 Promoter 의 특성

  • 유주현 (연세대학교 식품공학과) ;
  • 구본탁 (연세대학교 식품공학과) ;
  • 정용준 (연세대학교 식품공학과)
  • Published : 1989.06.01

Abstract

The promoters from akali-tolerant Bacillus sp. YA-14 chromosomal DMA cloned in B. subtilis using pPL703 were stably transferred to the donor strain. In alkali-tolerant Bacillus sp., the promoters revealed similiar properties with in B. subtilis but were preyed to be more efficient than in B. subtilis comparing with pPL708. Alkali-tolerant Bacillus sp. harboring the recombinant plasmid, p-l2Bl, was abnormally more inducible with chloramphenicol than B. subtilis haying the plasmid. Therefore the host-vector system using this recombinant plasmid and alkali-tolerant Bacillus sp. was expected to be more available.