Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Application of BMPI / HOBT Reagent in Solid-Phase Peptide Synthesis

  • Hong Nam Joo (Department of Applied Microbiology, College of Agriculture, Yeungnam University) ;
  • Choi Soo Kwan (Department of Chemistry, Korea University) ;
  • Koock Soon Uoong (Department of Chemistry, Korea University)
  • Published : 19890200
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Abstract

The suitability of BMPI (2-bromo-N-methyl pyridinium iodide) for solid-phase peptide synthesis was investigated. The coupling rate of BMPI/HOBT procedure. BMPI/HOBT was superior to DCC/HOBT couplings using the solid-phase peptide bond formation proceeded to a greater degree of completion than DCC/HOBT method did. Double couplings with 2 equiv. of Bocamino acids and 1.5 equiv. of BMPI and $NEt_3$ and 2 equiv. of HOBT in DMF/MC (1:1 v/v) gave the best result for the preparation of a model compound. Stepwise solid phase peptide synthesis using BMPI/HOBT procedure was successfully utilized for the preparation of $(D-Ala)^2$-dynorphine A. BMPI/HOBT procedure for the synthesis of $(D-Ala)^2$-dynorphine gave better yield (20%) than DCC/HOBT procedure did.

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References

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Cited by

  1. ChemInform Abstract: Application of BMPI/HOBT Reagent in Solid‐Phase Peptide Synthesis. vol.20, pp.31, 1989, https://doi.org/10.1002/chin.198931278