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Effects of SDS and Temperature on the Structural Changes of Bacteriorhodopsin

  • Lee In-Ja (Department of Chemistry, Korea University) ;
  • Lim Hyung-Sik (Department of Chemistry, Korea University) ;
  • Park Dong-Gon (Department of Chemistry, Korea University) ;
  • Kim Keon (Department of Chemistry, Korea University)
  • Published : 19890200

Abstract

Effects of SDS and temperature on the conformational changes of bacteriorhodopsin were studied using a, b, c bands of bacteriorhodopsin. In the SDS denaturation, bacteriorhodopsin in purple membrane was more labile than bacteriorhodopsin reconstituted into PC vesicles. These rather interesting results may be understood by effective SDS concentration in lipid layer.

Keywords

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