The Isolation and Evaluation of Bioactive Components from Crude Drugs against a Cariogenic Bacterium, Streptococcus mutans OMZ 176 (1) -On the Antibacterial Component of the Leaves of Liriodendron tulipifera and Its Safety-

충치균에 대한 생리활성 생약성분의 분리 및 약효평가(1) -튜립나무잎의 항균성 성분과 안전성에 대하여-

The isolation and identification of an antibacterial component, from the leaves of Liriodendron tulipifera. K. Kotch against a cariogenic bacterium Streptococcus mutans OMZ 176, were carried out for developing of anticariogenic agents. The bioactive component was elucidated as ${\beta}-liriodenolide$, which was isolated newly from the leaves of L. tulipifera. The minimal inhibitory concentration (MIC) of ${\beta}-liriodenolide$ was $100\;{\mu}g/ml$ and the antibacterial activity was stronger than that of berberine. ${\beta}-Liriodenolide$ inhibited ${\beta}-lactamase$ activity, 50, 100 and $200\;{\mu}M$ ${\beta}-liriodenolide$ did ${\beta}-lactamase$ activity as 0.7, 3.5 and 19.7%, respectively. The toxicity of ${\beta}-liriodenolide$ was not found with the method of photohemolysis.