Analysis of $[^3H]-Benzo(a)pyrene$ Metabolites by HPLC with Radioactive Flow Detection

Oh, Eun-Joo;Kim, Hyun-Pyo;Heo, Moon-Young;Kim, Kyeong-Ho;Park, Man-Ki;

YAKHAK HOEJI (약학회지)

Volume 34 Issue 5
/
Pages.291-295
/
1990
/
0377-9556(pISSN)
/
2383-9457(eISSN)

The Pharmaceutical Society of Korea (대한약학회)

Analysis of $[^3H]-Benzo(a)pyrene$ Metabolites by HPLC with Radioactive Flow Detection

Radio-HPLC에 의한 $[^3H]-Benzo(a)pyrene$

Oh, Eun-Joo (College of Pharmacy, Kangweon National University) ;
Kim, Hyun-Pyo (College of Pharmacy, Kangweon National University) ;
Heo, Moon-Young (College of Pharmacy, Kangweon National University) ;
Kim, Kyeong-Ho (College of Pharmacy, Kangweon National University) ;
Park, Man-Ki (College of Pharmacy, Seoul National University)

오은주 (강원대학교 약학대학) ;
김현표 (강원대학교 약학대학) ;
허문영 (강원대학교 약학대학) ;
김경호 (강원대학교 약학대학) ;
박만기 (서울대학교 약학대학)

Published : 1990.10.28

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Abstract

A modified method was investigated for the determination of benzo(a)pyrene metabolites generated by the rat liver microsomes based on the HPLC technique with radioactive flow detection. By adding $[^3H]-dexamethasone$ to the B(a)P metabolites mixture metabolized by the microsome, the poor yield of solvent extaction of B(a)P metabolites was compensated.

YAKHAK HOEJI (약학회지)

Analysis of $[^3H]-Benzo(a)pyrene$ Metabolites by HPLC with Radioactive Flow Detection

Radio-HPLC에 의한 $[^3H]-Benzo(a)pyrene$

Abstract

Keywords