Effect of Temperature and Nutrition Affecting Oospore Formation of Phytophthora capsici Causing Red Pepper Fruit Rot

고추 역병균(疫病菌) (Phytophthora capsici)의 난포자(卵胞子) 형성(形成)에 미치는 온도(溫度) 및 영양(營養)의 효과(效果)

  • Chung, Bong-Koo (Department of Agricultural Biology, College of Agriculture, Chung-buk National University) ;
  • Kang, Me-Jong (Department of Agricultural Biology, College of Agriculture, Chung-buk National University)
  • 정봉구 (충북대학교 농과대학 농생물학과) ;
  • 강미정 (충북대학교 농과대학 농생물학과)
  • Published : 1990.12.30

Abstract

Sexual reproductive structure of Phytophthora capsici in vitro was round shape with thick wall and $24.4\;{\mu}m$ of diameter ranging $20-32.5\;{\mu}m$. Oogonium was $26.7\;{\mu}m$ $(21-37.5\;{\mu}m$) and $6.5-{\times}5.4m$ $(6-8{\times}5-6{\mu}m)$ for antheridium as doughnut shaped. Since mycelial contact of the paired cultures initiated right after inoculation, mycelial expansion phase was followed. Oospore morphogensis could be divided into the four phases for reproducing adult oospores. The optimum temperature for oospore reproduction was $20-24^{\circ}C$, whereas a retard trend for oospore formation was at the temperature above $25^{\circ}C$. Korean squash agar medium showed a higher oospore formation than the existing V-8 agar medium. Red pepper fruit agar medium was next. No oospore was reproduced on the red pepper leaf medium. Diurnal light with $1800{\pm}300$ and $800{\pm}300$ Lux showed rather retardation for oospore formation than dark conditions.

고추 역병균(疫病菌) 유성세대(有性世代)인 난포자(卵胞子)는 원형(円形)이며 막(膜)이 두텁고 크기는 $24.4\;{\mu}m$$(20{\sim}32.5{\mu}m)$ 이며 장난기(藏卵器)는 $26.7\;{\mu}m$$(21{\sim}37.5{\mu}m)$이고 장정기(藏精器)는 도우낫모양으로 크기는 $6.5{\times}4\;{\mu}m(6{\sim}8{\times}5{\sim}6\;{\mu}m)$이었다. 난포자(卵胞子)의 형성단계(形成段階)는 접종(接種) 직후(直後) 각(各) 배우자형(配偶者型)의 균사(菌絲)가 신전(伸展)되어 접촉(接觸)되고 확장기(擴張期)로 이어지면서 완숙난포자(完熟卵胞子)로 되기까지 사단계(四段階)로 구분(區分)되었다. 난포자(卵胞子) 형성(形成)에 최적온도(最適溫度)는 $20{\sim}24^{\circ}C$이었고 온도(溫度)가 $25^{\circ}C$ 이상되면 오히려 감소(減少)하는 경향(傾向)이었다. 새로운 호박배지는 기존(旣存) V-8 한천배지(寒天培地)보다 많은 난포자(卵胞子) 형성(形成)을 보였으나 고추잎배지(培地)에서는 난포자(卵胞子)가 형성(形成)되지 않았다. $1800{\pm}300$$800{\pm}300$ Lux의 주야(晝夜) 광조사(光照射)는 암조건(暗條件)보다 오히려 난포자(卵胞子) 형성(形成)에 저해적(沮害的)이었다.

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